› 3h2c

X-ray diffraction
2.6Å resolution

Structural Studies of Pterin-Based Inhibitors of Dihydropteroate Synthase

Released:
DOI: 10.2210/pdb3h2c/pdb
PDB entry 3h2c coloured by chain, front view.
PDB entry 3h2c coloured by chain, side view.
PDB entry 3h2c coloured by chain, top view.
Source organism: Bacillus anthracis str. A2012
Primary publication:
Structural studies of pterin-based inhibitors of dihydropteroate synthase.
Hevener KE, Yun MK, Qi J, Kerr ID, Babaoglu K, Hurdle JG, Balakrishna K, White SW, Lee RE
J Med Chem 53 166-77 (2010)
PMID: 19899766

Function and Biology Details

Reaction catalysed: 
6-hydroxymethyl-7,8-dihydropterin diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182609 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydropteroate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 297 amino acids
Theoretical weight: 32.88 KDa
Source organism: Bacillus anthracis str. A2012
Expression system: Escherichia coli
UniProt:
  • Canonical: Q81VW8 (Residues: 4-280; Coverage: 99%)
Gene names: GBAA_0071, folP
Sequence domains: Pterin binding enzyme
Structure domains: Dihydropteroate synthase-like

Ligands and Environments

2 bound ligands:
Ligand B58 2 x B58
Ligand SO4 11 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P6222
Unit cell:
a: 97.667Å b: 97.667Å c: 263.574Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.249 0.246 0.298
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Utility of the Biosynthetic Folate Pathway for Targets in Antimicrobial Discovery.
Bourne CR. (2014)
7 more

1 mention without citation

Structural studies of pterin-based inhibitors of dihydropteroate synthase.
Hevener et al. (2010)