› 3gyi

X-ray diffraction
1Å resolution

Cholesterol oxidase from Streptomyces sp. N485D mutant (1.0A)

Released:
DOI: 10.2210/pdb3gyi/pdb
PDB entry 3gyi coloured by chain, front view.
PDB entry 3gyi coloured by chain, side view.
PDB entry 3gyi coloured by chain, top view.
Source organism: Streptomyces sp. SA-COO
Primary publication:
A hydrogen-bonding network is important for oxidation and isomerization in the reaction catalyzed by cholesterol oxidase.
Lyubimov AY, Chen L, Sampson NS, Vrielink A
Acta Crystallogr D Biol Crystallogr 65 1222-31 (2009)
PMID: 19923719

Function and Biology Details

Reactions catalysed: 
Cholesterol + O(2) = cholest-5-en-3-one + H(2)O(2)
A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146297 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cholesterol oxidase Chain: A
Molecule details ›
Chain: A
Length: 504 amino acids
Theoretical weight: 54.97 KDa
Source organism: Streptomyces sp. SA-COO
Expression system: Escherichia coli
UniProt:
  • Canonical: P12676 (Residues: 43-546; Coverage: 100%)
Gene name: choA
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21
Unit cell:
a: 51.276Å b: 72.783Å c: 62.97Å
α: 90° β: 105.12° γ: 90°
R-values:
R R work R free
0.126 0.126 0.158
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Cholesterol oxidase: biochemistry and structural features.
Vrielink et al. (2009)
4 other articles

3 mentions without citation

A hydrogen-bonding network is important for oxidation and isomerization in the reaction catalyzed by cholesterol oxidase.
Lyubimov et al. (2009)
2 more