› 3gu3

X-ray diffraction
2.3Å resolution

Crystal Structure of the methyltransferase BC_2162 in complex with S-Adenosyl-L-Homocysteine from Bacillus cereus, Northeast Structural Genomics Consortium Target BcR20

Released:
DOI: 10.2210/pdb3gu3/pdb
PDB entry 3gu3 coloured by chain, front view.
PDB entry 3gu3 coloured by chain, side view.
PDB entry 3gu3 coloured by chain, top view.
Source organism: Bacillus cereus ATCC 14579
Entry authors: Forouhar F, Neely H, Seetharaman J, Ciano C, Ma L, Zhao L, Everett JK, Nair R, Acton TB, Rost B, Montelione GT, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182498 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyltransferase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 284 amino acids
Theoretical weight: 32.94 KDa
Source organism: Bacillus cereus ATCC 14579
Expression system: Escherichia coli
UniProt:
  • Canonical: Q81E32 (Residues: 18-293; Coverage: 94%)
Gene name: BC_2162
Sequence domains: Methyltransferase domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ACT 2 x ACT
Ligand SAH 2 x SAH
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P32
Unit cell:
a: 90.793Å b: 90.793Å c: 122.627Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.184 0.212
Expression system: Escherichia coli

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