› 3gsi

X-ray diffraction
2Å resolution

Crystal structure of D552A dimethylglycine oxidase mutant of Arthrobacter globiformis in complex with tetrahydrofolate

Released:
DOI: 10.2210/pdb3gsi/pdb
PDB entry 3gsi coloured by chain, front view.
PDB entry 3gsi coloured by chain, side view.
PDB entry 3gsi coloured by chain, top view.
Source organism: Arthrobacter globiformis
Primary publication:
An internal reaction chamber in dimethylglycine oxidase provides efficient protection from exposure to toxic formaldehyde.
Tralau T, Lafite P, Levy C, Combe JP, Scrutton NS, Leys D
J Biol Chem 284 17826-34 (2009)
PMID: 19369258

Function and Biology Details

Reaction catalysed: 
N,N-dimethylglycine + H(2)O + O(2) = sarcosine + formaldehyde + H(2)O(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189547 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
2 bound ligands:
Ligand THG 1 x THG
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C222
Unit cell:
a: 71.215Å b: 224.548Å c: 119.382Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.153 0.15 0.202
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Structure, function, and mechanism of proline utilization A (PutA).
Liu et al. (2017)
3 more

2 mentions without citation

An internal reaction chamber in dimethylglycine oxidase provides efficient protection from exposure to toxic formaldehyde.
Tralau et al. (2009)
1 more