› 3geg

X-ray diffraction
2.1Å resolution

Fingerprint and Structural Analysis of a SCOR enzyme with its bound cofactor from Clostridium thermocellum

Released:
DOI: 10.2210/pdb3geg/pdb
PDB entry 3geg coloured by chain, front view.
PDB entry 3geg coloured by chain, side view.
PDB entry 3geg coloured by chain, top view.
Source organism: Acetivibrio thermocellus ATCC 27405
Primary publication:
Sequence fingerprint and structural analysis of the SCOR enzyme A3DFK9 from Clostridium thermocellum.
Huether R, Liu ZJ, Xu H, Wang BC, Pletnev VZ, Mao Q, Duax WL, Umland TC
Proteins 78 603-13 (2010)
PMID: 19774618

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107057 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Short-chain dehydrogenase/reductase SDR Chains: A, B
Molecule details ›
Chains: A, B
Length: 247 amino acids
Theoretical weight: 27.43 KDa
Source organism: Acetivibrio thermocellus ATCC 27405
Expression system: Escherichia coli
UniProt:
  • Canonical: A3DFK9 (Residues: 1-247; Coverage: 100%)
Gene name: Cthe_1510
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

4 bound ligands:
Ligand NAD 2 x NAD
Ligand GOL 2 x GOL
Ligand THJ 1 x THJ
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: I4122
Unit cell:
a: 124.272Å b: 124.272Å c: 161.208Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.167 0.2
Expression system: Escherichia coli

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Citations

1 citations in other articles

The short-chain oxidoreductase Q9HYA2 from Pseudomonas aeruginosa PAO1 contains an atypical catalytic center.
Huether et al. (2010)