› 3gaf

X-ray diffraction
2.2Å resolution

2.2A Crystal Structure of 7-Alpha-Hydroxysteroid Dehydrogenase from Brucella Melitensis

Released:
DOI: 10.2210/pdb3gaf/pdb
PDB entry 3gaf coloured by chain, front view.
PDB entry 3gaf coloured by chain, side view.
PDB entry 3gaf coloured by chain, top view.
Source organism: Brucella abortus 2308
Entry authors: Seattle Structural Genomics Center for Infectious Disease, Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholanate + NAD(+) = 3-alpha,12-alpha-dihydroxy-7-oxo-5-beta-cholanate + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187063 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
7-alpha-hydroxysteroid dehydrogenase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 256 amino acids
Theoretical weight: 26.34 KDa
Source organism: Brucella abortus 2308
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8YIN7 (Residues: 50-304; Coverage: 84%)
Gene name: BMEI0406
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 75.32Å b: 134.777Å c: 105.269Å
α: 90° β: 109.52° γ: 90°
R-values:
R R work R free
0.199 0.196 0.244
Expression system: Escherichia coli

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