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GR DNA binding domain:GilZ 16bp complex-65

X-ray diffraction
2.05Å resolution
PDB entry 3g8x coloured by chain, front view.
PDB entry 3g8x coloured by chain, side view.
PDB entry 3g8x coloured by chain, top view.
Source organism: Rattus norvegicus
Assembly composition:
hetero tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Glucocorticoid receptor and DNA
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Multimeric state: hetero tetramer
Accessible surface area: 12511.39 Å2
Buried surface area: 5732.56 Å2
Dissociation area: 2,677.22 Å2
Dissociation energy (ΔGdiss): 25.12 kcal/mol
Dissociation entropy (TΔSdiss): 31.6 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-116280

Macromolecules

Glucocorticoid receptor
Chains: A, B
Length: 90 amino acids
Theoretical weight: 9.96 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P06536 (Residues: 440-525; Coverage: 11%)
Gene names: Grl, Nr3c1
Pfam: Zinc finger, C4 type (two domains)
InterPro:
CATH: Erythroid Transcription Factor GATA-1, subunit A
Glucocorticoid receptor in PDB entry 3g8x, assembly 1, front view.
Glucocorticoid receptor in PDB entry 3g8x, assembly 1, side view.
Glucocorticoid receptor in PDB entry 3g8x, assembly 1, top view.
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DNA chain D
Name: DNA (5'-D(*TP*GP*GP*AP*AP*CP*CP*CP*AP*AP*TP*GP*TP*TP*CP*T)-3')
Representative chains: D
Source organism: Rattus norvegicus [10116]
Expression system: Not provided
Length: 16 nucleotides
Theoretical weight: 4.87 KDa
DNA (5'-D(*TP*GP*GP*AP*AP*CP*CP*CP*AP*AP*TP*GP*TP*TP*CP*T)-3') in PDB entry 3g8x, assembly 1, front view.
DNA (5'-D(*TP*GP*GP*AP*AP*CP*CP*CP*AP*AP*TP*GP*TP*TP*CP*T)-3') in PDB entry 3g8x, assembly 1, side view.
DNA (5'-D(*TP*GP*GP*AP*AP*CP*CP*CP*AP*AP*TP*GP*TP*TP*CP*T)-3') in PDB entry 3g8x, assembly 1, top view.
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DNA chain C
Name: DNA (5'-D(*AP*AP*GP*AP*AP*CP*AP*TP*TP*GP*GP*GP*TP*TP*CP*C)-3')
Representative chains: C
Source organism: Rattus norvegicus [10116]
Expression system: Not provided
Length: 16 nucleotides
Theoretical weight: 4.92 KDa
DNA (5'-D(*AP*AP*GP*AP*AP*CP*AP*TP*TP*GP*GP*GP*TP*TP*CP*C)-3') in PDB entry 3g8x, assembly 1, front view.
DNA (5'-D(*AP*AP*GP*AP*AP*CP*AP*TP*TP*GP*GP*GP*TP*TP*CP*C)-3') in PDB entry 3g8x, assembly 1, side view.
DNA (5'-D(*AP*AP*GP*AP*AP*CP*AP*TP*TP*GP*GP*GP*TP*TP*CP*C)-3') in PDB entry 3g8x, assembly 1, top view.
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