› 3g6t

X-ray diffraction
1.9Å resolution

GR gamma DNA-binding domain:FKBP5 16bp complex-34

Released:
DOI: 10.2210/pdb3g6t/pdb
PDB entry 3g6t coloured by chain, front view.
PDB entry 3g6t coloured by chain, side view.
PDB entry 3g6t coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
DNA binding site sequence directs glucocorticoid receptor structure and activity.
Meijsing SH, Pufall MA, So AY, Bates DL, Chen L, Yamamoto KR
Science 324 407-10 (2009)
PMID: 19372434

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116273 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Glucocorticoid receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 91 amino acids
Theoretical weight: 10.12 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P06536 (Residues: 440-470, 471-525; Coverage: 11%)
Gene names: Grl, Nr3c1
Sequence domains: Zinc finger, C4 type (two domains)
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
DNA (5'-D(*TP*AP*GP*AP*AP*CP*AP*CP*CP*CP*TP*GP*TP*TP*CP*T)-3') Chain: D
Molecule details ›
Chain: D
Length: 16 nucleotides
Theoretical weight: 4.83 KDa
DNA (5'-D(*AP*AP*GP*AP*AP*CP*AP*GP*GP*GP*TP*GP*TP*TP*CP*T)-3') Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.96 KDa

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2
Unit cell:
a: 115.693Å b: 39.716Å c: 95.228Å
α: 90° β: 122.79° γ: 90°
R-values:
R R work R free
0.181 0.179 0.214
Expression system: Escherichia coli

Quick links

Citations

121 review citations

Origins of specificity in protein-DNA recognition.
Rohs et al. (2010)
120 more

5 mentions without citation

Roles of Prolyl Isomerases in RNA-Mediated Gene Expression.
Thapar R. (2015)
4 more