› 3fz7

X-ray diffraction
2.5Å resolution

Crystal structure of apo glutamate decarboxylase beta from Escherichia coli

Released:
DOI: 10.2210/pdb3fz7/pdb
PDB entry 3fz7 coloured by chain, front view.
PDB entry 3fz7 coloured by chain, side view.
PDB entry 3fz7 coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Malashkevich VN, De Biase D, Bossa F

Function and Biology Details

Reaction catalysed: 
L-glutamate = 4-aminobutanoate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159688 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate decarboxylase beta Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 466 amino acids
Theoretical weight: 52.73 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P69910 (Residues: 1-466; Coverage: 100%)
Gene names: JW1488, b1493, gadB
Sequence domains: Pyridoxal-dependent decarboxylase conserved domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PO4 6 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P32
Unit cell:
a: 115.564Å b: 115.564Å c: 206.561Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.154 0.151 0.222
Expression system: Escherichia coli

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