› 3fs1

X-ray diffraction
2.2Å resolution

Crystal structure of HNF4a LBD in complex with the ligand and the coactivator PGC-1a fragment

Released:
DOI: 10.2210/pdb3fs1/pdb
PDB entry 3fs1 coloured by chain, front view.
PDB entry 3fs1 coloured by chain, side view.
PDB entry 3fs1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Multiple binding modes between HNF4alpha and the LXXLL motifs of PGC-1alpha lead to full activation.
Rha GB, Wu G, Shoelson SE, Chi YI
J Biol Chem 284 35165-76 (2009)
PMID: 19846556

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154349 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Hepatocyte nuclear factor 4-alpha Chain: A
Molecule details ›
Chain: A
Length: 230 amino acids
Theoretical weight: 25.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P41235 (Residues: 148-377; Coverage: 49%)
Gene names: HNF4, HNF4A, NR2A1, TCF14
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
PPARgamma Coactivator-1a (PGC-1a) Chain: B
Molecule details ›
Chain: B
Length: 9 amino acids
Theoretical weight: 784 Da
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand MYR 1 x MYR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P4222
Unit cell:
a: 113.049Å b: 113.049Å c: 57.322Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.19 0.236
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Classic and Novel Sex Hormone Binding Globulin Effects on the Cardiovascular System in Men.
Basualto-Alarcón et al. (2021)
13 other articles

12 mentions without citation

Identification of the Flavonoid Luteolin as a Repressor of the Transcription Factor Hepatocyte Nuclear Factor 4α.
Li et al. (2015)
11 more