3fra Summary


Staphylococcus aureus F98Y DHFR complexed with iclaprim

The structure was published by Oefner, C., Bandera, M., Haldimann, A., et al., Weiss, L., Lociuro, S., and Dale, G.E., in 2009 in a paper entitled "Increased hydrophobic interactions of iclaprim with Staphylococcus aureus dihydrofolate reductase are responsible for the increase in affinity and antibacterial activity" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.35 Å and deposited in 2009.

The experimental data on which the structure is based was also deposited.

The PDB entry contains the structure of Dihydrofolate reductase. This molecule has the UniProt identifier P0A017 (DYR_STAAU)search. The sample contained 158 residues which is 99% of the natural sequence. Out of 158 residues 157 were observed and are deposited in the PDB.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule is most likely monomeric.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
X Dihydrofolate reductase P0A017 (2-159) (DYR_STAAU)search Staphylococcus aureussearch 99% 158 99%

This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
P0A017 (2 - 159) Dihydrofolate reductase Staphylococcus aureus

Chain Structural classification (CATH) Sequence family (Pfam)
X (P0A017) Dihydrofolate Reductase, subunit Asearch PF00186: Dihydrofolate reductasesearch

Chain ID Molecular function (GO) Biological process (GO)
X (P0A017) dihydrofolate reductase activitysearch oxidoreductase activitysearch NADP bindingsearch nucleotide biosynthetic processsearch glycine biosynthetic processsearch oxidation-reduction processsearch one-carbon metabolic processsearch tetrahydrofolate biosynthetic processsearch

Chain InterPro annotation
X Dihydrofolate reductase domainsearch Dihydrofolate reductasesearch Dihydrofolate reductase conserved sitesearch Dihydrofolate reductase-like domainsearch