› 3fmz

X-ray diffraction
2.9Å resolution

Crystal Structure of Retinol-Binding Protein 4 (RBP4) in complex with non-retinoid ligand

Released:
DOI: 10.2210/pdb3fmz/pdb
PDB entry 3fmz coloured by chain, front view.
PDB entry 3fmz coloured by chain, side view.
PDB entry 3fmz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Identification and characterization of a non-retinoid ligand for retinol-binding protein 4 which lowers serum retinol-binding protein 4 levels in vivo.
Motani A, Wang Z, Conn M, Siegler K, Zhang Y, Liu Q, Johnstone S, Xu H, Thibault S, Wang Y, Fan P, Connors R, Le H, Xu G, Walker N, Shan B, Coward P
J Biol Chem 284 7673-80 (2009)
PMID: 19147488

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136146 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Retinol-binding protein 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 212 amino acids
Theoretical weight: 24.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P02753 (Residues: 19-201; Coverage: 100%)
Gene names: PRO2222, RBP4
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

1 bound ligand:
Ligand 2T1 2 x 2T1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P65
Unit cell:
a: 84.802Å b: 84.802Å c: 119.557Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.223 0.286
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Signaling by vitamin A and retinol-binding protein in regulation of insulin responses and lipid homeostasis.
Berry et al. (2012)
14 more

9 mentions without citation

Lipocalin 2 (LCN2) Expression in Hepatic Malfunction and Therapy.
Asimakopoulou et al. (2016)
8 more