3fjn

X-ray diffraction
2.3Å resolution

The crystal structure of 17-alpha hydroxysteroid dehydrogenase Y224D mutant.

Released:

Function and Biology Details

Reaction catalysed:
Androsterone + NAD(P)(+) = 5-alpha-androstane-3,17-dione + NAD(P)H
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-187581 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aldo-keto reductase family 1 member C21 Chains: A, B
Molecule details ›
Chains: A, B
Length: 323 amino acids
Theoretical weight: 36.88 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q91WR5 (Residues: 1-323; Coverage: 100%)
Gene name: Akr1c21
Sequence domains: Aldo/keto reductase family
Structure domains: NADP-dependent oxidoreductase domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21212
Unit cell:
a: 95.317Å b: 97.837Å c: 69.61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.174 0.277
Expression system: Escherichia coli