3fhi Summary


PDB entry 3fhi (supersedes 1u7e)

Crystal structure of a complex between the catalytic and regulatory (RI{alpha}) subunits of PKA

The structure was published by Kim, C., Xuong, N.H., and Taylor, S.S., in 2005 in a paper entitled "Crystal structure of a complex between the catalytic and regulatory (RIalpha) subunits of PKA." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.0 Å and deposited in 2008.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely cAMP-dependent protein kinase catalytic subunit alpha and cAMP-dependent protein kinase type I-alpha regulatory subunit.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms heterodimers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A cAMP-dependent protein kinase catalytic subunit alpha P05132 (2-351) (KAPCA_MOUSE)search Mus musculussearch 96% 350 96%
B cAMP-dependent protein kinase type I-alpha regulatory subunit P00514 (92-245) (KAP0_BOVIN)search Bos taurussearch 100% 154 97%

This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P05132 (2 - 351) cAMP-dependent protein kinase catalytic subunit alpha Mus musculus
P00514 (92 - 245) cAMP-dependent protein kinase type I-alpha regulatory subunit Bos taurus

Chain Structural classification (CATH) Sequence family (Pfam)
A (P05132) Transferase(Phosphotransferase) domain 1search, Phosphorylase Kinase; domain 1search PF00069: Protein kinase domainsearch
B (P00514) Jelly Rollssearch PF00027: Cyclic nucleotide-binding domainsearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A (P05132) ATP bindingsearch protein kinase activitysearch protein serine/threonine kinase activitysearch kinase activitysearch cAMP-dependent protein kinase activitysearch ubiquitin protein ligase bindingsearch protein bindingsearch protein kinase bindingsearch transferase activitysearch protein kinase A regulatory subunit bindingsearch nucleotide bindingsearch protein serine/threonine/tyrosine kinase activitysearch transferase activity, transferring phosphorus-containing groupssearch protein phosphorylationsearch cellular response to parathyroid hormone stimulussearch regulation of osteoblast differentiationsearch protein autophosphorylationsearch regulation of tight junction assemblysearch regulation of proteasomal protein catabolic processsearch regulation of synaptic transmissionsearch mesoderm formationsearch cellular response to glucose stimulussearch regulation of protein processingsearch sperm capacitationsearch peptidyl-threonine phosphorylationsearch phosphorylationsearch negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterningsearch positive regulation of protein export from nucleussearch peptidyl-serine phosphorylationsearch positive regulation of cell cycle arrestsearch neural tube closuresearch cytoplasmsearch nucleussearch nucleoplasmsearch AMP-activated protein kinase complexsearch cytosolsearch plasma membranesearch extracellular vesicular exosomesearch ciliumsearch mitochondrionsearch motile ciliumsearch centrosomesearch sperm midpiecesearch cell projectionsearch membranesearch ciliary basesearch neuromuscular junctionsearch
B (P00514) cAMP-dependent protein kinase regulator activitysearch regulation of protein phosphorylationsearch cAMP-dependent protein kinase complexsearch

Chain InterPro annotation
A Protein kinase domainsearch AGC-kinase, C-terminalsearch Serine/threonine/dual specificity protein kinase, catalytic domainsearch Serine/threonine-protein kinase, active sitesearch Protein kinase-like domainsearch Protein kinase, ATP binding sitesearch
B Cyclic nucleotide-binding domainsearch cAMP/cGMP-dependent protein kinasesearch RmlC-like jelly roll foldsearch Cyclic nucleotide-binding, conserved sitesearch Cyclic nucleotide-binding-likesearch