3fhi Summary


PDB entry 3fhi (supersedes 1u7e)

Crystal structure of a complex between the catalytic and regulatory (RI{alpha}) subunits of PKA

The structure was published by Kim, C., Xuong, N.H., and Taylor, S.S., in 2005 in a paper entitled "Crystal structure of a complex between the catalytic and regulatory (RIalpha) subunits of PKA." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.0 Å and deposited in 2008.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely cAMP-dependent protein kinase catalytic subunit alpha and cAMP-dependent protein kinase type I-alpha regulatory subunit.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms heterodimers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A cAMP-dependent protein kinase catalytic subunit alpha P05132 (2-351) (KAPCA_MOUSE)search Mus musculussearch 96% 350 96%
B cAMP-dependent protein kinase type I-alpha regulatory subunit P00514 (92-245) (KAP0_BOVIN)search Bos taurussearch 100% 154 97%

This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P05132 (2 - 351) cAMP-dependent protein kinase catalytic subunit alpha Mus musculus
P00514 (92 - 245) cAMP-dependent protein kinase type I-alpha regulatory subunit Bos taurus

Chain Structural classification (CATH) Sequence family (Pfam)
A (P05132) Transferase(Phosphotransferase) domain 1search, Phosphorylase Kinase; domain 1search PF00069: Protein kinase domainsearch
B (P00514) Jelly Rollssearch PF00027: Cyclic nucleotide-binding domainsearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A (P05132) ATP bindingsearch protein serine/threonine kinase activitysearch protein kinase activitysearch protein kinase bindingsearch cAMP-dependent protein kinase activitysearch transferase activitysearch protein serine/threonine/tyrosine kinase activitysearch protein bindingsearch kinase activitysearch nucleotide bindingsearch ubiquitin protein ligase bindingsearch transferase activity, transferring phosphorus-containing groupssearch protein kinase A regulatory subunit bindingsearch protein phosphorylationsearch protein autophosphorylationsearch regulation of proteasomal protein catabolic processsearch cellular response to glucose stimulussearch cellular response to parathyroid hormone stimulussearch phosphorylationsearch regulation of synaptic transmissionsearch mesoderm formationsearch regulation of protein processingsearch sperm capacitationsearch peptidyl-threonine phosphorylationsearch negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterningsearch regulation of tight junction assemblysearch positive regulation of cell cycle arrestsearch regulation of osteoblast differentiationsearch neural tube closuresearch peptidyl-serine phosphorylationsearch positive regulation of protein export from nucleussearch nucleoplasmsearch cytosolsearch cytoplasmsearch plasma membranesearch extracellular vesicular exosomesearch centrosomesearch AMP-activated protein kinase complexsearch nucleussearch mitochondrionsearch membranesearch ciliary basesearch motile ciliumsearch cell projectionsearch ciliumsearch neuromuscular junctionsearch sperm midpiecesearch
B (P00514) cAMP-dependent protein kinase regulator activitysearch regulation of protein phosphorylationsearch cAMP-dependent protein kinase complexsearch

Chain InterPro annotation
A Protein kinase domainsearch AGC-kinase, C-terminalsearch Serine/threonine/dual specificity protein kinase, catalytic domainsearch Serine/threonine-protein kinase, active sitesearch Protein kinase-like domainsearch Protein kinase, ATP binding sitesearch
B Cyclic nucleotide-binding domainsearch cAMP/cGMP-dependent protein kinasesearch RmlC-like jelly roll foldsearch Cyclic nucleotide-binding, conserved sitesearch Cyclic nucleotide-binding-likesearch