› 3fg3

X-ray diffraction
1.9Å resolution

Crystal structure of Delta413-417:GS I805W LOX

Released:
DOI: 10.2210/pdb3fg3/pdb
PDB entry 3fg3 coloured by chain, front view.
PDB entry 3fg3 coloured by chain, side view.
PDB entry 3fg3 coloured by chain, top view.
Source organism: Plexaura homomalla
Primary publication:
The 1.85 A structure of an 8R-lipoxygenase suggests a general model for lipoxygenase product specificity.
Neau DB, Gilbert NC, Bartlett SG, Boeglin W, Brash AR, Newcomer ME
Biochemistry 48 7906-15 (2009)
PMID: 19594169

Function and Biology Details

Reaction catalysed: 
Arachidonate + O(2) = (5Z,9E,11Z,14Z)-(8R)-8-hydroperoxyicosa-5,9,11,14-tetraenoate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-127557 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Allene oxide synthase-lipoxygenase protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 696 amino acids
Theoretical weight: 79.48 KDa
Source organism: Plexaura homomalla
Expression system: Escherichia coli
UniProt:
  • Canonical: O16025 (Residues: 374-1066; Coverage: 65%)
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand FE2 4 x FE2
Ligand CA 8 x CA
Ligand ACY 23 x ACY
Ligand GOL 21 x GOL
Ligand CL 6 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CAMD BEAMLINE GCPCC
Spacegroup: P21
Unit cell:
a: 103.704Å b: 170.214Å c: 104.202Å
α: 90° β: 96.13° γ: 90°
R-values:
R R work R free
0.164 0.162 0.206
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Lipoxygenase and leukotriene pathways: biochemistry, biology, and roles in disease.
Haeggström et al. (2011)
11 more

2 mentions without citation

The 1.85 A structure of an 8R-lipoxygenase suggests a general model for lipoxygenase product specificity.
Neau et al. (2009)
1 more