› 3f7d

X-ray diffraction
2.2Å resolution

SF-1 LBD bound by phosphatidylcholine

Released:
DOI: 10.2210/pdb3f7d/pdb
PDB entry 3f7d coloured by chain, front view.
PDB entry 3f7d coloured by chain, side view.
PDB entry 3f7d coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structure of SF-1 bound by different phospholipids: evidence for regulatory ligands.
Sablin EP, Blind RD, Krylova IN, Ingraham JG, Cai F, Williams JD, Fletterick RJ, Ingraham HA
Mol Endocrinol 23 25-34 (2009)
PMID: 18988706

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130691 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Steroidogenic factor 1 Chain: A
Molecule details ›
Chain: A
Length: 244 amino acids
Theoretical weight: 27.97 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P33242 (Residues: 219-462; Coverage: 53%)
Gene names: Ftzf1, Nr5a1
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
Peroxisome proliferator-activated receptor gamma coactivator 1-alpha Chain: B
Molecule details ›
Chain: B
Length: 14 amino acids
Theoretical weight: 1.52 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: O70343 (Residues: 137-150; Coverage: 2%)
Gene names: Pgc1, Pgc1a, Ppargc1, Ppargc1a

Ligands and Environments

1 bound ligand:
Ligand P42 1 x P42
1 modified residue:
Ligand CAF 1 x CAF

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 46.895Å b: 67.228Å c: 82.689Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.241
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

19 review citations

Minireview: steroidogenic factor 1: its roles in differentiation, development, and disease.
Schimmer et al. (2010)
18 more

4 mentions without citation

Disentangling biological signaling networks by dynamic coupling of signaling lipids to modifying enzymes.
Blind RD. (2014)
3 more