› 3f6x

X-ray diffraction
2.35Å resolution

c-Src kinase domain in complex with small molecule inhibitor

Released:
DOI: 10.2210/pdb3f6x/pdb
PDB entry 3f6x coloured by chain, front view.
PDB entry 3f6x coloured by chain, side view.
PDB entry 3f6x coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Tuning a three-component reaction for trapping kinase substrate complexes.
Statsuk AV, Maly DJ, Seeliger MA, Fabian MA, Biggs WH, Lockhart DJ, Zarrinkar PP, Kuriyan J, Shokat KM
J Am Chem Soc 130 17568-74 (2008)
PMID: 19053485

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132629 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proto-oncogene tyrosine-protein kinase Src Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 286 amino acids
Theoretical weight: 32.73 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00523 (Residues: 251-533; Coverage: 53%)
Gene name: SRC
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand IHH 4 x IHH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P1
Unit cell:
a: 63.697Å b: 74.488Å c: 84.652Å
α: 89.06° β: 89.97° γ: 78.43°
R-values:
R R work R free
0.234 0.234 0.266
Expression system: Escherichia coli

Quick links

Citations

10 review citations

The genesis of tyrosine phosphorylation.
Hunter T. (2014)
9 more

6 mentions without citation

Three-Dimensional Interactions Analysis of the Anticancer Target c-Src Kinase with Its Inhibitors.
Jha et al. (2020)
5 more