› 3f6t

X-ray diffraction
2.15Å resolution

Crystal structure of aspartate aminotransferase (E.C. 2.6.1.1) (YP_194538.1) from Lactobacillus acidophilus NCFM at 2.15 A resolution

Released:
DOI: 10.2210/pdb3f6t/pdb
PDB entry 3f6t coloured by chain, front view.
PDB entry 3f6t coloured by chain, side view.
PDB entry 3f6t coloured by chain, top view.
Source organism: Lactobacillus acidophilus NCFM
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177097 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminotransferase class I/classII domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 533 amino acids
Theoretical weight: 61.27 KDa
Source organism: Lactobacillus acidophilus NCFM
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5FIG7 (Residues: 1-532; Coverage: 100%)
Gene name: LBA1695
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand EDO 5 x EDO
2 modified residues:
Ligand MSE 18 x MSE
Ligand LLP 2 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P212121
Unit cell:
a: 89.78Å b: 107.75Å c: 119.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.177 0.238
Expression system: Escherichia coli

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