› 3f1o

X-ray diffraction
1.6Å resolution

Crystal structure of the high affinity heterodimer of HIF2 alpha and ARNT C-terminal PAS domains, with an internally-bound artificial ligand

Released:
DOI: 10.2210/pdb3f1o/pdb
PDB entry 3f1o coloured by chain, front view.
PDB entry 3f1o coloured by chain, side view.
PDB entry 3f1o coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Artificial ligand binding within the HIF2alpha PAS-B domain of the HIF2 transcription factor.
Scheuermann TH, Tomchick DR, Machius M, Guo Y, Bruick RK, Gardner KH
Proc Natl Acad Sci U S A 106 450-5 (2009)
PMID: 19129502

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151026 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Endothelial PAS domain-containing protein 1 Chain: A
Molecule details ›
Chain: A
Length: 117 amino acids
Theoretical weight: 13.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99814 (Residues: 239-350; Coverage: 13%)
Gene names: BHLHE73, EPAS1, HIF2A, MOP2, PASD2
Sequence domains: PAS domain
Structure domains: PAS domain
Aryl hydrocarbon receptor nuclear translocator Chain: B
Molecule details ›
Chain: B
Length: 121 amino acids
Theoretical weight: 14.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P27540 (Residues: 356-470; Coverage: 15%)
Gene names: ARNT, BHLHE2
Sequence domains: PAS domain
Structure domains: PAS domain

Ligands and Environments

2 bound ligands:
Ligand 2XY 1 x 2XY
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: C2
Unit cell:
a: 73.761Å b: 83.019Å c: 41.553Å
α: 90° β: 106.36° γ: 90°
R-values:
R R work R free
0.168 0.166 0.203
Expression system: Escherichia coli

Quick links

Citations

49 review citations

VHL, the story of a tumour suppressor gene.
Gossage et al. (2015)
48 more

23 mentions without citation

Structure and signaling mechanism of Per-ARNT-Sim domains.
Möglich et al. (2009)
22 more