› 3f0o

X-ray diffraction
1.76Å resolution

Crystal structure of MerB, the Organomercurial Lyase involved in a bacterial mercury resistance system

Released:
DOI: 10.2210/pdb3f0o/pdb
PDB entry 3f0o coloured by chain, front view.
PDB entry 3f0o coloured by chain, side view.
PDB entry 3f0o coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structures of the organomercurial lyase MerB in its free and mercury-bound forms: insights into the mechanism of methylmercury degradation.
Lafrance-Vanasse J, Lefebvre M, Di Lello P, Sygusch J, Omichinski JG
J Biol Chem 284 938-44 (2009)
PMID: 19004822

Function and Biology Details

Reaction catalysed: 
An alkylmercury + H(+) = an alkane + Hg(2+)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160120 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alkylmercury lyase Chains: A, B
Molecule details ›
Chains: A, B
Length: 212 amino acids
Theoretical weight: 23.06 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P77072 (Residues: 1-212; Coverage: 100%)
Gene name: merB
Sequence domains:
Structure domains: Beta-Lactamase

Ligands and Environments

1 bound ligand:
Ligand BR 1 x BR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P21
Unit cell:
a: 38.71Å b: 89.947Å c: 52.167Å
α: 90° β: 100.53° γ: 90°
R-values:
R R work R free
0.183 0.182 0.208
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Bacterial mer operon-mediated detoxification of mercurial compounds: a short review.
Mathema et al. (2011)
1 more

1 mention without citation

Structural and Biochemical Characterization of Organotin and Organolead Compounds Binding to the Organomercurial Lyase MerB Provide New Insights into Its Mechanism of Carbon-Metal Bond Cleavage.
Wahba et al. (2017)