› 3ez4

X-ray diffraction
2.1Å resolution

Crystal structure of 3-methyl-2-oxobutanoate hydroxymethyltransferase from Burkholderia pseudomallei

Released:
DOI: 10.2210/pdb3ez4/pdb
PDB entry 3ez4 coloured by chain, front view.
PDB entry 3ez4 coloured by chain, side view.
PDB entry 3ez4 coloured by chain, top view.
Source organism: Burkholderia pseudomallei
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H(2)O = tetrahydrofolate + 2-dehydropantoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174651 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-methyl-2-oxobutanoate hydroxymethyltransferase Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 269 amino acids
Theoretical weight: 28.54 KDa
Source organism: Burkholderia pseudomallei
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3JP15 (Residues: 12-271; Coverage: 96%)
Gene names: BURPS1710b_3317, panB
Sequence domains: Ketopantoate hydroxymethyltransferase
Structure domains: Phosphoenolpyruvate-binding domains

Ligands and Environments

1 bound ligand:
Ligand TRS 5 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21212
Unit cell:
a: 173.784Å b: 187.556Å c: 83.493Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.195 0.244
Expression system: Escherichia coli

Quick links