› 3en9

X-ray diffraction
2.67Å resolution

Structure of the Methanococcus jannaschii KAE1-BUD32 fusion protein

Released:
DOI: 10.2210/pdb3en9/pdb
PDB entry 3en9 coloured by chain, front view.
PDB entry 3en9 coloured by chain, side view.
PDB entry 3en9 coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
Atomic structure of the KEOPS complex: an ancient protein kinase-containing molecular machine.
Mao DY, Neculai D, Downey M, Orlicky S, Haffani YZ, Ceccarelli DF, Ho JS, Szilard RK, Zhang W, Ho CS, Wan L, Fares C, Rumpel S, Kurinov I, Arrowsmith CH, Durocher D, Sicheri F
Mol Cell 32 259-75 (2008)
PMID: 18951093

Function and Biology Details

Reactions catalysed: 
L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176639 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 540 amino acids
Theoretical weight: 61.13 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58530 (Residues: 1-535; Coverage: 100%)
Gene name: MJ1130
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand TBR 2 x TBR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P4122
Unit cell:
a: 148.73Å b: 148.73Å c: 136.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.202 0.264
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Protein Ser/Thr/Tyr phosphorylation in the Archaea.
Kennelly PJ. (2014)
4 more

7 mentions without citation

The structural and functional workings of KEOPS.
Beenstock et al. (2021)
6 more