3eid Summary

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CDK2/CyclinA complexed with a pyrazolopyridazine inhibitor

The structure was published by Stevens, K.L., Reno, M.J., Alberti, J.B., et al., Davis, S.T., Griffin, R.J., and Peel, M.R., in 2008 in a paper entitled "Synthesis and evaluation of pyrazolo[1,5-b]pyridazines as selective cyclin dependent kinase inhibitors." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 3.15 Å and deposited in 2008.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely Cell division protein kinase 2 and Cyclin-A2.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Cell division protein kinase 2 P24941 (1-298) (CDK2_HUMAN)search Homo sapienssearch 97% 298 97%
C Cell division protein kinase 2 P24941 (1-298) (CDK2_HUMAN)search Homo sapienssearch 97% 298 97%
B Cyclin-A2 P20248 (173-432) (CCNA2_HUMAN)search Homo sapienssearch < 90% 260 98%
D Cyclin-A2 P20248 (173-432) (CCNA2_HUMAN)search Homo sapienssearch < 90% 260 98%


This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P24941 (1 - 298) Cell division protein kinase 2 Homo sapiens
P20248 (173 - 432) Cyclin-A2 Homo sapiens

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A, C (P24941) Phosphorylase Kinase; domain 1search, Transferase(Phosphotransferase) domain 1search PF00069: Protein kinase domainsearch
B, D Cyclinsearch Cyclin A; domain 1search Cyclin, N-terminal domainsearch, Cyclin, C-terminal domainsearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A, C (P24941) protein serine/threonine kinase activitysearch ATP bindingsearch protein kinase activitysearch protein bindingsearch cyclin-dependent protein serine/threonine kinase activitysearch kinase activitysearch transferase activity, transferring phosphorus-containing groupssearch cyclin bindingsearch metal ion bindingsearch nucleotide bindingsearch histone kinase activitysearch transferase activitysearch protein phosphorylationsearch DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrestsearch positive regulation of transcription, DNA-templatedsearch DNA repairsearch Ras protein signal transductionsearch mitotic cell cyclesearch phosphorylationsearch anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic processsearch G1/S transition of mitotic cell cyclesearch centrosome duplicationsearch cellular response to nitric oxidesearch G2/M transition of mitotic cell cyclesearch mitotic nuclear divisionsearch blood coagulationsearch cellular response to DNA damage stimulussearch histone phosphorylationsearch regulation of ubiquitin-protein ligase activity involved in mitotic cell cyclesearch potassium ion transportsearch meiotic nuclear divisionsearch cell divisionsearch DNA replicationsearch positive regulation of DNA-dependent DNA replication initiationsearch cell cyclesearch mitotic G1 DNA damage checkpointsearch positive regulation of cell proliferationsearch regulation of gene silencingsearch nucleoplasmsearch endosomesearch cytoplasmsearch chromosome, telomeric regionsearch nucleussearch cytosolsearch Cajal bodysearch X chromosomesearch cytoskeletonsearch microtubule organizing centersearch cyclin-dependent protein kinase holoenzyme complexsearch centrosomesearch transcription factor complexsearch Y chromosomesearch condensed chromosomesearch
B, D (P20248) protein kinase bindingsearch regulation of cyclin-dependent protein serine/threonine kinase activitysearch regulation of G2/M transition of mitotic cell cyclesearch regulation of cell cyclesearch nucleussearch

Chain InterPro annotation
A, C Protein kinase domainsearch Serine/threonine/dual specificity protein kinase, catalytic domainsearch Serine/threonine-protein kinase, active sitesearch Protein kinase-like domainsearch Protein kinase, ATP binding sitesearch
B, D Cyclin, C-terminal domainsearch Cyclin, N-terminalsearch Cyclin-likesearch Cyclin A/B/D/E/Fsearch Cyclin A, chordatessearch