› 3egi

X-ray diffraction
2.21Å resolution

Methyltransferase domain of human trimethylguanosine synthase TGS1 bound to m7GpppA (inactive form)

Released:
DOI: 10.2210/pdb3egi/pdb
PDB entry 3egi coloured by chain, front view.
PDB entry 3egi coloured by chain, side view.
PDB entry 3egi coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure analysis of the conserved methyltransferase domain of human trimethylguanosine synthase TGS1.
Monecke T, Dickmanns A, Strasser A, Ficner R
Acta Crystallogr D Biol Crystallogr 65 332-8 (2009)
PMID: 19307714

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188738 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Trimethylguanosine synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 206 amino acids
Theoretical weight: 22.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96RS0 (Residues: 653-853; Coverage: 24%)
Gene names: HCA137, NCOA6IP, PIMT, TGS1
Sequence domains: RNA cap guanine-N2 methyltransferase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

1 bound ligand:
Ligand ADP 4 x ADP
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P43212
Unit cell:
a: 213.893Å b: 213.893Å c: 62.415Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.252
Expression system: Escherichia coli

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Citations

1 review citation

Enzymology of RNA cap synthesis.
Ghosh et al. (2010)
3 other articles

3 mentions without citation

RNA methyltransferases involved in 5' cap biosynthesis.
Byszewska et al. (2014)
2 more