› 3e9k

X-ray diffraction
1.7Å resolution

Crystal structure of Homo sapiens kynureninase-3-hydroxyhippuric acid inhibitor complex

Released:
DOI: 10.2210/pdb3e9k/pdb
PDB entry 3e9k coloured by chain, front view.
PDB entry 3e9k coloured by chain, side view.
PDB entry 3e9k coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the Homo sapiens kynureninase-3-hydroxyhippuric acid inhibitor complex: insights into the molecular basis of kynureninase substrate specificity.
Lima S, Kumar S, Gawandi V, Momany C, Phillips RS
J Med Chem 52 389-96 (2009)
PMID: 19143568

Function and Biology Details

Reaction catalysed: 
L-kynurenine + H(2)O = anthranilate + L-alanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172562 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kynureninase Chain: A
Molecule details ›
Chain: A
Length: 465 amino acids
Theoretical weight: 52.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16719 (Residues: 1-465; Coverage: 100%)
Gene name: KYNU
Sequence domains: Aminotransferase class-V
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
1 bound ligand:
Ligand 3XH 1 x 3XH
1 modified residue:
Ligand CSX 1 x CSX

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 74.444Å b: 77.124Å c: 94.558Å
α: 90° β: 109.35° γ: 90°
R-values:
R R work R free
0.155 0.153 0.191
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Kynurenines in the CNS: recent advances and new questions.
Vécsei et al. (2013)
8 more

5 mentions without citation

Biallelic variants in KYNU cause a multisystemic syndrome with hand hyperphalangism.
Ehmke et al. (2020)
4 more