› 3e8s

X-ray diffraction
2.1Å resolution

Crystal structure of Putative SAM Dependent Methyltransferase in Complex with SAH (NP_744700.1) from PSEUDOMONAS PUTIDA KT2440 at 2.10 A resolution

Released:
DOI: 10.2210/pdb3e8s/pdb
PDB entry 3e8s coloured by chain, front view.
PDB entry 3e8s coloured by chain, side view.
PDB entry 3e8s coloured by chain, top view.
Source organism: Pseudomonas putida KT2440
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183482 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyltransferase type 12 Chain: A
Molecule details ›
Chain: A
Length: 227 amino acids
Theoretical weight: 25.24 KDa
Source organism: Pseudomonas putida KT2440
Expression system: Escherichia coli
UniProt:
  • Canonical: Q88JU2 (Residues: 1-226; Coverage: 100%)
Gene name: PP_2555
Sequence domains: Methyltransferase domain
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

1 bound ligand:
Ligand SAH 1 x SAH
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P64
Unit cell:
a: 130.433Å b: 130.433Å c: 54.431Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.159 0.158 0.176
Expression system: Escherichia coli

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