› 3e6e

X-ray diffraction
2.5Å resolution

Crystal structure of Alanine racemase from E.faecalis complex with cycloserine

Released:
DOI: 10.2210/pdb3e6e/pdb
PDB entry 3e6e coloured by chain, front view.
PDB entry 3e6e coloured by chain, side view.
PDB entry 3e6e coloured by chain, top view.
Source organism: Enterococcus faecalis
Primary publication:
Structural insights into the alanine racemase from Enterococcus faecalis.
Priyadarshi A, Lee EH, Sung MW, Nam KH, Lee WH, Kim EE, Hwang KY
Biochim Biophys Acta 1794 1030-40 (2009)
PMID: 19328247

Function and Biology Details

Reaction catalysed: 
L-alanine = D-alanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182840 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alanine racemase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 371 amino acids
Theoretical weight: 41.03 KDa
Source organism: Enterococcus faecalis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q837J0 (Residues: 1-371; Coverage: 100%)
Gene names: EF_0849, alr
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand DCS 3 x DCS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: C2221
Unit cell:
a: 94.597Å b: 156.186Å c: 146.93Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.21 0.26
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Inhibitors of alanine racemase enzyme: a review.
Azam et al. (2016)
9 other articles

3 mentions without citation

Inhibition of the PLP-dependent enzyme serine palmitoyltransferase by cycloserine: evidence for a novel decarboxylative mechanism of inactivation.
Lowther et al. (2010)
2 more