› 3e4y

X-ray diffraction
2.6Å resolution

Crystal structure of a 33kDa catalase-related protein from Mycobacterium avium subsp. paratuberculosis. I2(1)2(1)2(1) crystal form

Released:
DOI: 10.2210/pdb3e4y/pdb
PDB entry 3e4y coloured by chain, front view.
PDB entry 3e4y coloured by chain, side view.
PDB entry 3e4y coloured by chain, top view.
Source organism: Mycobacterium avium subsp. paratuberculosis
Primary publication:
The structure and peroxidase activity of a 33-kDa catalase-related protein from Mycobacterium avium ssp. paratuberculosis.
Pakhomova S, Gao B, Boeglin WE, Brash AR, Newcomer ME
Protein Sci 18 2559-68 (2009)
PMID: 19827095

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180977 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Catalase-related peroxidase Chain: A
Molecule details ›
Chain: A
Length: 320 amino acids
Theoretical weight: 34.32 KDa
Source organism: Mycobacterium avium subsp. paratuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q73WB6 (Residues: 1-313; Coverage: 100%)
Gene name: MAP_2744c
Sequence domains: Catalase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand HEM 1 x HEM
Ligand GOL 2 x GOL
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CAMD BEAMLINE GCPCC
Spacegroup: I212121
Unit cell:
a: 69.604Å b: 92.68Å c: 165.499Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.202 0.257
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Thirty years of heme catalases structural biology.
Díaz et al. (2012)
6 other articles

1 mention without citation

The structure and peroxidase activity of a 33-kDa catalase-related protein from Mycobacterium avium ssp. paratuberculosis.
Pakhomova et al. (2009)