› › Structure analysis

Q138F HincII bound to GTCGAC and Ca2+ (cocrystallized)

X-ray diffraction
2.68Å resolution
PDB entry 3e42 coloured by chain, front view.
PDB entry 3e42 coloured by chain, side view.
PDB entry 3e42 coloured by chain, top view.
Source organism: Haemophilus influenzae
Assembly composition:
hetero tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule
1 distinct DNA molecule

Quick links

Assemblies

Assembly 1 (preferred)
Download    3D Visualisation
Assembly Name: Type II restriction enzyme HincII and DNA
hide full assembly name
Multimeric state: hetero tetramer
Accessible surface area: 22725.78 Å2
Buried surface area: 9779.79 Å2
Dissociation area: 2,880.09 Å2
Dissociation energy (ΔGdiss): 75.27 kcal/mol
Dissociation entropy (TΔSdiss): 14.08 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-116197

Macromolecules

Type II restriction enzyme HincII
Chains: A, B
Length: 257 amino acids
Theoretical weight: 29.84 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P17743 (Residues: 2-258; Coverage: 100%)
Gene name: hincIIR
Pfam: Restriction endonuclease HincII
InterPro:
CATH: DNA mismatch repair MutH/Restriction endonuclease, type II
Type II restriction enzyme HincII in PDB entry 3e42, assembly 1, front view.
Type II restriction enzyme HincII in PDB entry 3e42, assembly 1, side view.
Type II restriction enzyme HincII in PDB entry 3e42, assembly 1, top view.
Molecule details ›

Search similar proteins

DNA chains E, F
Name: 5'-D(*DGP*DCP*DCP*DGP*DGP*DTP*DCP*DGP*DAP*DCP*DCP*DGP*DGP*DC)-3'
Representative chains: E, F
Source organism: Haemophilus influenzae [727]
Expression system: Not provided
Length: 14 nucleotides
Theoretical weight: 4.28 KDa
5'-D(*DGP*DCP*DCP*DGP*DGP*DTP*DCP*DGP*DAP*DCP*DCP*DGP*DGP*DC)-3' in PDB entry 3e42, assembly 1, front view.
5'-D(*DGP*DCP*DCP*DGP*DGP*DTP*DCP*DGP*DAP*DCP*DCP*DGP*DGP*DC)-3' in PDB entry 3e42, assembly 1, side view.
5'-D(*DGP*DCP*DCP*DGP*DGP*DTP*DCP*DGP*DAP*DCP*DCP*DGP*DGP*DC)-3' in PDB entry 3e42, assembly 1, top view.
Molecule details ›

Search similar DNA

Quick links