3e3y Summary


Q138F HincII bound to GTTAAC and cocrystallized with 5 mM Ca2+

The structure was published by Babic, A.C., Little, E.J., Manohar, V.M., Bitinaite, J., and Horton, N.C., in 2008 in a paper entitled "DNA distortion and specificity in a sequence-specific endonuclease." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.13 Å and deposited in 2008.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely Type-2 restriction enzyme HindII and 5'-D(*DGP*DCP*DCP*DGP*DGP*DTP*DTP*DAP*DAP*DCP*DCP*DGP*DGP*DC)-3'.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms heterotetramers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Type-2 restriction enzyme HindII P17743 (2-258) (T2C2_HAEIF)search Haemophilus influenzaesearch 100% 257 94%
B Type-2 restriction enzyme HindII P17743 (2-258) (T2C2_HAEIF)search Haemophilus influenzaesearch 100% 257 94%

This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
P17743 (2 - 258) Type-2 restriction enzyme HindII Haemophilus influenzae

Chain Structural classification (CATH) Sequence family (Pfam)
A, B (P17743) ECO RV Endonuclease; Chain Asearch PF09226: Restriction endonuclease HincIIsearch

Chain ID Biological process (GO) Molecular function (GO)
A, B (P17743) DNA restriction-modification systemsearch nucleic acid phosphodiester bond hydrolysissearch DNA catabolic process, endonucleolyticsearch DNA bindingsearch Type II site-specific deoxyribonuclease activitysearch hydrolase activitysearch endonuclease activitysearch nuclease activitysearch

Chain InterPro annotation
A, B Restriction endonuclease type II-likesearch DNA mismatch repair MutH/Restriction endonuclease, type IIsearch Restriction endonuclease, type II, HincIIsearch