Structure analysis

H. influenzae beta-carbonic anhydrase, variant G41A with 100 mM bicarbonate

X-ray diffraction
2Å resolution
Source organism: Haemophilus influenzae
Assembly composition:
homo tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule

Assemblies

Assembly 1 (preferred)
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Multimeric state: homo tetramer
Accessible surface area: 32335.21 Å2
Buried surface area: 18034.67 Å2
Dissociation area: 2,358.58 Å2
Dissociation energy (ΔGdiss): 56.88 kcal/mol
Dissociation entropy (TΔSdiss): 14.31 kcal/mol
Symmetry number: 4
PDBe Complex ID: PDB-CPX-155253
Assembly 2
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Multimeric state: homo tetramer
Accessible surface area: 32566.8 Å2
Buried surface area: 18135.74 Å2
Dissociation area: 6,408.42 Å2
Dissociation energy (ΔGdiss): 104.31 kcal/mol
Dissociation entropy (TΔSdiss): 15.64 kcal/mol
Symmetry number: 4
PDBe Complex ID: PDB-CPX-155253
Assembly 3
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Multimeric state: homo tetramer
Accessible surface area: 32061.64 Å2
Buried surface area: 17649.07 Å2
Dissociation area: 6,168.29 Å2
Dissociation energy (ΔGdiss): 96.49 kcal/mol
Dissociation entropy (TΔSdiss): 15.55 kcal/mol
Symmetry number: 4
PDBe Complex ID: PDB-CPX-155253

Macromolecules

Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 229 amino acids
Theoretical weight: 26.3 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P45148 (Residues: 1-229; Coverage: 100%)
Gene names: HI_1301, can
Pfam: Carbonic anhydrase
InterPro:
CATH: Carbonic anhydrase

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