› 3e2w

X-ray diffraction
2.3Å resolution

H. influenzae beta-carbonic anhydrase, variant Y181F with 1M bicarbonate

Released:
DOI: 10.2210/pdb3e2w/pdb
PDB entry 3e2w coloured by chain, front view.
PDB entry 3e2w coloured by chain, side view.
PDB entry 3e2w coloured by chain, top view.
Source organism: Haemophilus influenzae
Primary publication:
Allosteric site variants of Haemophilus influenzae beta-carbonic anhydrase.
Rowlett RS, Tu C, Lee J, Herman AG, Chapnick DA, Shah SH, Gareiss PC
Biochemistry 48 6146-56 (2009)
PMID: 19459702

Function and Biology Details

Reaction catalysed: 
H(2)CO(3) = CO(2) + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155253 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbonic anhydrase 2 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 229 amino acids
Theoretical weight: 26.27 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P45148 (Residues: 1-229; Coverage: 100%)
Gene names: HI_1301, can
Sequence domains: Carbonic anhydrase
Structure domains: Carbonic anhydrase

Ligands and Environments

3 bound ligands:
Ligand ZN 6 x ZN
Ligand SO4 12 x SO4
Ligand BCT 1 x BCT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 250.141Å b: 145.225Å c: 53.499Å
α: 90° β: 93.78° γ: 90°
R-values:
R R work R free
0.207 0.205 0.242
Expression system: Escherichia coli

Quick links

Citations

1 review citation

An Overview of the Bacterial Carbonic Anhydrases.
Supuran et al. (2017)
11 other articles