› 3drd

X-ray diffraction
2.17Å resolution

Crystal Structure of 7,8 Diaminopelargonic Acid Synthase Apoenzyme in Bacillus subtilis

Released:
DOI: 10.2210/pdb3drd/pdb
PDB entry 3drd coloured by chain, front view.
PDB entry 3drd coloured by chain, side view.
PDB entry 3drd coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Structural characterization of the Mycobacterium tuberculosis biotin biosynthesis enzymes 7,8-diaminopelargonic acid synthase and dethiobiotin synthetase .
Dey S, Lane JM, Lee RE, Rubin EJ, Sacchettini JC
Biochemistry 49 6746-60 (2010)
PMID: 20565114

Function and Biology Details

Reaction catalysed: 
L-lysine + 8-amino-7-oxononanoate = (S)-2-amino-6-oxohexanoate + 7,8-diaminononanoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156809 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-Lysine--8-amino-7-oxononanoate transaminase Chains: A, B
Molecule details ›
Chains: A, B
Length: 448 amino acids
Theoretical weight: 50.17 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P53555 (Residues: 1-448; Coverage: 100%)
Gene names: BSU30230, bioA, bioK
Sequence domains: Aminotransferase class-III
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 58.173Å b: 102.844Å c: 74.555Å
α: 90° β: 105.15° γ: 90°
R-values:
R R work R free
0.208 0.205 0.265
Expression system: Escherichia coli

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Citations

7 review citations

Closing in on complete pathways of biotin biosynthesis.
Lin et al. (2011)
6 more

4 mentions without citation

A Survey of Pyridoxal 5'-Phosphate-Dependent Proteins in the Gram-Positive Model Bacterium Bacillus subtilis.
Richts et al. (2019)
3 more