› 3dpj

X-ray diffraction
1.9Å resolution

The crystal structure of a TetR transcription regulator from Silicibacter pomeroyi DSS

Released:
DOI: 10.2210/pdb3dpj/pdb
PDB entry 3dpj coloured by chain, front view.
PDB entry 3dpj coloured by chain, side view.
PDB entry 3dpj coloured by chain, top view.
Source organism: Ruegeria pomeroyi
Entry authors: Tan K, Li H, Freeman L, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177555 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH tetR-type domain-containing protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 194 amino acids
Theoretical weight: 21.92 KDa
Source organism: Ruegeria pomeroyi
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q5LS67 (Residues: 1-191; Coverage: 100%)
Gene name: SPO1902
Sequence domains: Bacterial regulatory proteins, tetR family
Structure domains: Tetracycline Repressor, domain 2

Ligands and Environments

2 bound ligands:
Ligand MES 8 x MES
Ligand EDO 30 x EDO
1 modified residue:
Ligand MSE 56 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P1
Unit cell:
a: 60.523Å b: 93.072Å c: 93.397Å
α: 89.35° β: 71.02° γ: 71.04°
R-values:
R R work R free
0.196 0.193 0.243
Expression system: Escherichia coli BL21

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