› 3dkx

X-ray diffraction
2.7Å resolution

Crystal Structure of the replication initiator protein encoded on plasmid pMV158 (RepB), trigonal form, to 2.7 Ang resolution

Released:
DOI: 10.2210/pdb3dkx/pdb
PDB entry 3dkx coloured by chain, front view.
PDB entry 3dkx coloured by chain, side view.
PDB entry 3dkx coloured by chain, top view.
Source organism: Streptococcus agalactiae
Primary publication:
Plasmid replication initiator RepB forms a hexamer reminiscent of ring helicases and has mobile nuclease domains.
Boer DR, Ruíz-Masó JA, López-Blanco JR, Blanco AG, Vives-Llàcer M, Chacón P, Usón I, Gomis-Rüth FX, Espinosa M, Llorca O, del Solar G, Coll M
EMBO J 28 1666-78 (2009)
PMID: 19440202

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146815 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Replication protein RepB Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 210 amino acids
Theoretical weight: 24.29 KDa
Source organism: Streptococcus agalactiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P13921 (Residues: 1-210; Coverage: 100%)
Gene name: repB
Sequence domains: Plasmid replication protein
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MN 3 x MN
Ligand CL 3 x CL
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29, ESRF BEAMLINE ID14-4, ESRF BEAMLINE BM14, RIGAKU
Spacegroup: P3221
Unit cell:
a: 85.611Å b: 85.611Å c: 245.53Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.23 0.228 0.276
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The Different Faces of Rolling-Circle Replication and Its Multifunctional Initiator Proteins.
Wawrzyniak et al. (2017)
1 more

7 mentions without citation

The Facts and Family Secrets of Plasmids That Replicate via the Rolling-Circle Mechanism.
Garcillán-Barcia et al. (2022)
6 more