3dhw Summary

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Crystal structure of methionine importer MetNI

The structure was published by Kadaba, N.S., Kaiser, J.T., Johnson, E., Lee, A., and Rees, D.C., in 2008 in a paper entitled "The high-affinity E. coli methionine ABC transporter: structure and allosteric regulation." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 3.7 Å and deposited in 2008.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely D-methionine transport system permease protein metI and Methionine import ATP-binding protein metN.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A D-methionine transport system permease protein metI P31547 (1-217) (METI_ECOLI)search Escherichia coli K-12search 100% 217 93%
B D-methionine transport system permease protein metI P31547 (1-217) (METI_ECOLI)search Escherichia coli K-12search 100% 217 93%
E D-methionine transport system permease protein metI P31547 (1-217) (METI_ECOLI)search Escherichia coli K-12search 100% 217 93%
F D-methionine transport system permease protein metI P31547 (1-217) (METI_ECOLI)search Escherichia coli K-12search 100% 217 93%
C Methionine import ATP-binding protein metN P30750 (1-343) (METN_ECOLI)search Escherichia coli K-12search 100% 343 100%
D Methionine import ATP-binding protein metN P30750 (1-343) (METN_ECOLI)search Escherichia coli K-12search 100% 343 100%
G Methionine import ATP-binding protein metN P30750 (1-343) (METN_ECOLI)search Escherichia coli K-12search 100% 343 100%
H Methionine import ATP-binding protein metN P30750 (1-343) (METN_ECOLI)search Escherichia coli K-12search 100% 343 100%


This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P31547 (1 - 217) D-methionine transport system permease protein metI Escherichia coli K-12
P30750 (1 - 343) Methionine import ATP-binding protein metN Escherichia coli K-12

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A, B, E, F (P31547) MetI-likesearch MetI-like domainsearch PF00528: Binding-protein-dependent transport system inner membrane componentsearch
C, D, G, H (P30750) ABC transporter ATPase domain-likesearch, NIL domain-likesearch P-loop containing nucleotide triphosphate hydrolasessearch, Alpha-Beta Plaitssearch PF00005: ABC transportersearch, PF09383: NIL domainsearch

Chain ID Biological process (GO) Cellular component (GO) Molecular function (GO)
A, B, E, F (P31547) transportsearch amino acid transportsearch membranesearch integral component of membranesearch plasma membranesearch protein bindingsearch
C, D, G, H (P30750) methionine transportsearch D-methionine transportsearch transmembrane transportsearch metabolic processsearch amino acid transportsearch transportsearch amino acid transmembrane transportsearch ATP catabolic processsearch Gram-negative-bacterium-type cell wallsearch plasma membranesearch ATP-binding cassette (ABC) transporter complexsearch membranesearch ATP bindingsearch ATPase activitysearch D-methionine transmembrane transporter activitysearch ATPase activity, coupled to transmembrane movement of substancessearch nucleotide bindingsearch protein bindingsearch hydrolase activitysearch amino acid-transporting ATPase activitysearch

Chain InterPro annotation
A, B, E, F MetI-like domainsearch
C, D, G, H ABC transporter-likesearch AAA+ ATPase domainsearch ABC transporter, methionine import, ATP-binding protein MetN, proteobacteriasearch ABC transporter, conserved sitesearch ABC transporter, methionine import, ATP-binding protein, MetN, C-terminalsearch NIL domainsearch Methionine import ATP-binding protein MetNsearch P-loop containing nucleoside triphosphate hydrolasesearch