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PDBe Entry: 3df4 view

Crystal structure of the bacterial ribosome from Escherichia coli in complex with hygromycin B. This file contains the 50S subunit of the second 70S ribosome. The entire crystal structure contains two 70S ribosomes.
Summary
Header RIBOSOMEsearch
Method X-RAY DIFFRACTION
Experiment Resolution: 3.5 Å, R-factor: 26.9%, Free R-factor: 31.8%, Spacegroup: P 21 21 21
Released 19/08/2008, deposition: 11/06/2008, last revision: 03/11/2009
Authors Borovinskaya, M.A.search; Shoji, S.search; Fredrick, K.search; Cate, J.H.D.search
Primary citation Structural basis for hygromycin B inhibition of protein biosynthesis
RNAsearch vol:14, pag:1590-1599 (2008) [PubMed ID 18567815 ]search
Keywords RNA-protein complexsearch, RIBOSOMEsearch
Organism Escherichia coli 562search(A B V C D E F G H J K L M N O P Q R S T U W X Y Z 0 1 2 3 4 I)
UniProt 50S ribosomal protein L25 P68919search (V)
50S ribosomal protein L2 P60422search (C)
50S ribosomal protein L3 P60438search (D)
50S ribosomal protein L4 P60723search (E)
50S ribosomal protein L5 P62399search (F)
50S ribosomal protein L6 P0AG57search (G)
50S ribosomal protein L9 P0A7R1search (H)
50S ribosomal protein L13 P0AA10search (J)
50S ribosomal protein L14 P0ADY5search (K)
50S ribosomal protein L15 P02413search (L)
50S ribosomal protein L16 P0ADY9search (M)
50S ribosomal protein L17 P0AG46search (N)
50S ribosomal protein L18 P0C018search (O)
50S ribosomal protein L19 P0A7K6search (P)
50S ribosomal protein L20 P0A7L3search (Q)
50S ribosomal protein L21 P0AG49search (R)
50S ribosomal protein L22 P61175search (S)
50S ribosomal protein L23 P0ADZ2search (T)
50S ribosomal protein L24 P60624search (U)
50S ribosomal protein L27 P0A7L8search (W)
50S ribosomal protein L29 P0A7M6search (X)
50S ribosomal protein L30 P0AG53search (Y)
50S ribosomal protein L28 P0A7M2search (Z)
50S ribosomal protein L32 P0A7N4search (0)
50S ribosomal protein L33 P0A7N9search (1)
50S ribosomal protein L34 P0A7P5search (2)
50S ribosomal protein L35 (Ribosomal protein A) P0A7Q1search (3)
50S ribosomal protein L36 (Ribosomal protein B) P0A7Q6search (4)
50S ribosomal protein L11 P0A7J7search (I)
Solvent 2, B, C, D, L
Related entries 3df1, 3df2, 3df3, 3df4
Polymers
Id Name Type UniProt Residues Observed
A 5S ribosomal RNA RNA
120 97%
B 23S ribosomal RNA RNA
2904 97%
V 50S ribosomal protein L25 Protein P68919 (RL25_ECOLI)search
94 100%
C 50S ribosomal protein L2 Protein P60422 (RL2_ECOLI)search
273 99%
D 50S ribosomal protein L3 Protein P60438 (RL3_ECOLI)search
209 100%
E 50S ribosomal protein L4 Protein P60723 (RL4_ECOLI)search
201 100%
F 50S ribosomal protein L5 Protein P62399 (RL5_ECOLI)search
178 100%
G 50S ribosomal protein L6 Protein P0AG57 (RL6_ECO57)search
176 100%
H 50S ribosomal protein L9 Protein P0A7R1 (RL9_ECOLI)search
149 100%
J 50S ribosomal protein L13 Protein P0AA10 (RL13_ECOLI)search
142 100%
K 50S ribosomal protein L14 Protein P0ADY5 (RL14_ECO57)search
123 98%
L 50S ribosomal protein L15 Protein P02413 (RL15_ECOLI)search
144 99%
M 50S ribosomal protein L16 Protein P0ADY9 (RL16_ECO57)search
136 100%
N 50S ribosomal protein L17 Protein P0AG46 (RL17_ECO57)search
127 94%
O 50S ribosomal protein L18 Protein P0C018 (RL18_ECOLI)search
117 99%
P 50S ribosomal protein L19 Protein P0A7K6 (RL19_ECOLI)search
114 100%
Q 50S ribosomal protein L20 Protein P0A7L3 (RL20_ECOLI)search
117 100%
R 50S ribosomal protein L21 Protein P0AG49 (RL21_ECO57)search
103 100%
S 50S ribosomal protein L22 Protein P61175 (RL22_ECOLI)search
110 100%
T 50S ribosomal protein L23 Protein P0ADZ2 (RL23_ECO57)search
100 93%
U 50S ribosomal protein L24 Protein P60624 (RL24_ECOLI)search
103 99%
W 50S ribosomal protein L27 Protein P0A7L8 (RL27_ECOLI)search
84 94%
X 50S ribosomal protein L29 Protein P0A7M6 (RL29_ECOLI)search
63 100%
Y 50S ribosomal protein L30 Protein P0AG53 (RL30_ECO57)search
58 100%
Z 50S ribosomal protein L28 Protein P0A7M2 (RL28_ECOLI)search
78 98%
0 50S ribosomal protein L32 Protein P0A7N4 (RL32_ECOLI)search
56 100%
1 50S ribosomal protein L33 Protein P0A7N9 (RL33_ECOLI)search
54 92%
2 50S ribosomal protein L34 Protein P0A7P5 (RL34_ECOLI)search
46 100%
3 50S ribosomal protein L35 Protein P0A7Q1 (RL35_ECOLI)search
64 100%
4 50S ribosomal protein L36 Protein P0A7Q6 (RL36_ECOLI)search
38 100%
I 50S ribosomal protein L11 Protein P0A7J7 (RL11_ECOLI)search
141 100%
Heterogens
Id Name Ligands
B MAGNESIUM ION MG search
4 ZINC ION ZN search
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