3ddq Summary


Structure of phosphorylated Thr160 CDK2/cyclin A in complex with the inhibitor roscovitine

The structure was published by Bettayeb, K., Oumata, N., Echalier, A., et al., Endicott, J.A., Galons, H., and Meijer, L., in 2008 in a paper entitled "CR8, a potent and selective, roscovitine-derived inhibitor of cyclin-dependent kinases." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 1.8 Å and deposited in 2008.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely Cell division protein kinase 2 and Cyclin-A2.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Cell division protein kinase 2 P24941 (1-298) (CDK2_HUMAN)search Homo sapienssearch 97% 299 99%
C Cell division protein kinase 2 P24941 (1-298) (CDK2_HUMAN)search Homo sapienssearch 97% 299 99%
B Cyclin-A2 P30274 (169-430) (CCNA2_BOVIN)search Bos taurussearch < 90% 269 100%
D Cyclin-A2 P30274 (169-430) (CCNA2_BOVIN)search Bos taurussearch < 90% 269 100%

This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P24941 (1 - 298) Cell division protein kinase 2 Homo sapiens
P30274 (169 - 430) Cyclin-A2 Bos taurus

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A, C (P24941) Phosphorylase Kinase; domain 1search, Transferase(Phosphotransferase) domain 1search PF00069: Protein kinase domainsearch
B, D Cyclinsearch Cyclin A; domain 1search Cyclin, N-terminal domainsearch, Cyclin, C-terminal domainsearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A, C (P24941) protein kinase activitysearch transferase activity, transferring phosphorus-containing groupssearch ATP bindingsearch protein bindingsearch cyclin bindingsearch cyclin-dependent protein serine/threonine kinase activitysearch histone kinase activitysearch protein serine/threonine kinase activitysearch transferase activitysearch metal ion bindingsearch kinase activitysearch nucleotide bindingsearch protein phosphorylationsearch cell divisionsearch DNA replicationsearch G2/M transition of mitotic cell cyclesearch centrosome duplicationsearch Ras protein signal transductionsearch regulation of gene silencingsearch potassium ion transportsearch mitotic cell cyclesearch meiotic nuclear divisionsearch phosphorylationsearch positive regulation of cell proliferationsearch positive regulation of DNA-dependent DNA replication initiationsearch histone phosphorylationsearch mitotic G2 phasesearch blood coagulationsearch DNA repairsearch regulation of ubiquitin-protein ligase activity involved in mitotic cell cyclesearch cellular response to nitric oxidesearch mitotic nuclear divisionsearch positive regulation of transcription, DNA-templatedsearch anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic processsearch G1/S transition of mitotic cell cyclesearch mitotic G1 DNA damage checkpointsearch DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrestsearch cellular response to DNA damage stimulussearch cell cyclesearch nucleoplasmsearch cytoskeletonsearch endosomesearch cyclin-dependent protein kinase holoenzyme complexsearch cytosolsearch nucleussearch cytoplasmsearch chromosome, telomeric regionsearch microtubule organizing centersearch X chromosomesearch transcription factor complexsearch Y chromosomesearch Cajal bodysearch condensed chromosomesearch centrosomesearch
B, D (P30274) protein kinase bindingsearch regulation of cyclin-dependent protein serine/threonine kinase activitysearch regulation of G2/M transition of mitotic cell cyclesearch regulation of cell cyclesearch nucleussearch

Chain InterPro annotation
A, C Protein kinase domainsearch Serine/threonine/dual specificity protein kinase, catalytic domainsearch Serine/threonine-protein kinase, active sitesearch Protein kinase-like domainsearch Protein kinase, ATP binding sitesearch
B, D Cyclin, C-terminal domainsearch Cyclin, N-terminalsearch Cyclin-likesearch Cyclin A/B/D/E/Fsearch Cyclin A, chordatessearch