› 3da8

X-ray diffraction
1.3Å resolution

Crystal structure of PurN from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb3da8/pdb
PDB entry 3da8 coloured by chain, front view.
PDB entry 3da8 coloured by chain, side view.
PDB entry 3da8 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Structures of glycinamide ribonucleotide transformylase (PurN) from Mycobacterium tuberculosis reveal a novel dimer with relevance to drug discovery.
Zhang Z, Caradoc-Davies TT, Dickson JM, Baker EN, Squire CJ
J Mol Biol 389 722-33 (2009)
PMID: 19394344

Function and Biology Details

Reaction catalysed: 
10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161566 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoribosylglycinamide formyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 215 amino acids
Theoretical weight: 22.41 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WHM5 (Residues: 2-215; Coverage: 100%)
Gene names: Rv0956, purN
Sequence domains: Formyl transferase
Structure domains: Formyl transferase, N-terminal domain

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand IOD 9 x IOD
Ligand BME 1 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 73.273Å b: 73.366Å c: 77.246Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.164 0.186
Expression system: Escherichia coli

Quick links

Citations

5 citation in other articles

Biological and structural evaluation of 10R- and 10S-methylthio-DDACTHF reveals a new role for sulfur in inhibition of glycinamide ribonucleotide transformylase.
Connelly et al. (2013)
4 more

4 mentions without citation

Principles and characteristics of biological assemblies in experimentally determined protein structures.
Xu et al. (2019)
3 more