› 3d8g

X-ray diffraction
1.99Å resolution

Crystal structure of Staphylococcal nuclease variant Delta+PHS I72R at cryogenic temperature

Released:
DOI: 10.2210/pdb3d8g/pdb
PDB entry 3d8g coloured by chain, front view.
PDB entry 3d8g coloured by chain, side view.
PDB entry 3d8g coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus MW2
Primary publication:
Arginine residues at internal positions in a protein are always charged.
Harms MJ, Schlessman JL, Sue GR, García-Moreno B
Proc Natl Acad Sci U S A 108 18954-9 (2011)
PMID: 22080604

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185690 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thermonuclease Chain: A
Molecule details ›
Chain: A
Length: 143 amino acids
Theoretical weight: 16.19 KDa
Source organism: Staphylococcus aureus subsp. aureus MW2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NXI6 (Residues: 80-228; Coverage: 70%)
Gene names: MW0769, nuc
Sequence domains: Staphylococcal nuclease homologue
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand THP 1 x THP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P212121
Unit cell:
a: 32.181Å b: 60.425Å c: 73.124Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.2 0.258
Expression system: Escherichia coli

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Citations

11 review citations

Peptide-Based Molecular Hydrogels as Supramolecular Protein Mimics.
Singh et al. (2017)
10 more

1 mention without citation

Arginine residues at internal positions in a protein are always charged.
Harms et al. (2011)