3d87 Summary

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Crystal structure of Interleukin-23

The structure was published by Beyer, B.M., Ingram, R., Ramanathan, L., et al., Le, H.V., Madison, V., and Orth, P., in 2008 in a paper entitled "Crystal structures of the pro-inflammatory cytokine interleukin-23 and its complex with a high-affinity neutralizing antibody" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.9 Å and deposited in 2008.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely Interleukin-23 subunit p19 and Interleukin-12 subunit p40.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Interleukin-23 subunit p19 Q9NPF7 (20-189) (IL23A_HUMAN)search Homo sapienssearch 100% 178 90%
C Interleukin-23 subunit p19 Q9NPF7 (20-189) (IL23A_HUMAN)search Homo sapienssearch 100% 178 90%
B Interleukin-12 subunit p40 P29460 (23-328) (IL12B_HUMAN)search Homo sapienssearch 100% 306 97%
D Interleukin-12 subunit p40 P29460 (23-328) (IL12B_HUMAN)search Homo sapienssearch 100% 306 97%


This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
Q9NPF7 (20 - 189) Interleukin-23 subunit p19 Homo sapiens
P29460 (23 - 328) Interleukin-12 subunit p40 Homo sapiens

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A, C (Q9NPF7) PF00489: Interleukin-6/G-CSF/MGF familysearch
B, D (P29460) I set domainssearch, Fibronectin type IIIsearch Immunoglobulinssearch PF10420: Cytokine interleukin-12p40 C-terminussearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A, C (Q9NPF7) cytokine activitysearch protein bindingsearch interleukin-23 receptor bindingsearch positive regulation of T-helper 17 type immune responsesearch positive regulation of NF-kappaB import into nucleussearch positive regulation of neutrophil chemotaxissearch innate immune responsesearch positive regulation of tyrosine phosphorylation of Stat4 proteinsearch positive regulation of interleukin-12 productionsearch positive regulation of NK T cell activationsearch positive regulation of interleukin-17 productionsearch positive regulation of interferon-gamma productionsearch positive regulation of defense response to virus by hostsearch immune responsesearch positive regulation of interleukin-10 productionsearch defense response to Gram-negative bacteriumsearch negative regulation of interleukin-10 productionsearch positive regulation of tumor necrosis factor productionsearch T cell proliferationsearch positive regulation of T cell mediated cytotoxicitysearch defense response to virussearch positive regulation of T-helper 1 type immune responsesearch positive regulation of activation of JAK2 kinase activitysearch positive regulation of memory T cell differentiationsearch positive regulation of tissue remodelingsearch positive regulation of granulocyte macrophage colony-stimulating factor productionsearch positive regulation of natural killer cell proliferationsearch immune system processsearch positive regulation of tyrosine phosphorylation of Stat5 proteinsearch positive regulation of tyrosine phosphorylation of Stat3 proteinsearch positive regulation of activated T cell proliferationsearch regulation of tyrosine phosphorylation of Stat1 proteinsearch tissue remodelingsearch positive regulation of T cell proliferationsearch positive regulation of natural killer cell activationsearch positive regulation of NK T cell proliferationsearch positive regulation of transcription from RNA polymerase II promotersearch inflammatory responsesearch positive regulation of inflammatory responsesearch positive regulation of osteoclast differentiationsearch positive regulation of T-helper 17 cell lineage commitmentsearch interleukin-23 complexsearch extracellular spacesearch extracellular regionsearch
B, D (P29460) interleukin-12 alpha subunit bindingsearch protein bindingsearch cytokine activitysearch protein heterodimerization activitysearch interleukin-23 receptor bindingsearch cytokine receptor activitysearch identical protein bindingsearch cytokine receptor bindingsearch growth factor activitysearch interleukin-12 receptor bindingsearch protein homodimerization activitysearch cell cycle arrestsearch positive regulation of T-helper 1 type immune responsesearch positive regulation of tyrosine phosphorylation of Stat4 proteinsearch positive regulation of defense response to virus by hostsearch positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell targetsearch natural killer cell activation involved in immune responsesearch defense response to protozoansearch positive regulation of interferon-gamma biosynthetic processsearch positive regulation of tissue remodelingsearch positive regulation of natural killer cell activationsearch response to UV-Bsearch positive regulation of T cell mediated cytotoxicitysearch natural killer cell activationsearch positive regulation of cell adhesionsearch positive regulation of T-helper 17 cell lineage commitmentsearch positive regulation of interferon-gamma productionsearch positive regulation of activation of JAK2 kinase activitysearch positive regulation of interleukin-17 productionsearch cellular response to lipopolysaccharidesearch T-helper 1 type immune responsesearch cellular response to interferon-gammasearch positive regulation of mononuclear cell proliferationsearch positive regulation of inflammatory responsesearch regulation of cytokine biosynthetic processsearch positive regulation of natural killer cell proliferationsearch positive regulation of NK T cell proliferationsearch positive regulation of T cell proliferationsearch T-helper cell differentiationsearch positive regulation of activated T cell proliferationsearch regulation of tyrosine phosphorylation of Stat1 proteinsearch positive regulation of interleukin-12 productionsearch positive regulation of interleukin-10 productionsearch cell migrationsearch sexual reproductionsearch positive regulation of granulocyte macrophage colony-stimulating factor productionsearch positive regulation of memory T cell differentiationsearch positive regulation of lymphocyte proliferationsearch negative regulation of growth of symbiont in hostsearch positive regulation of osteoclast differentiationsearch defense response to virussearch cell surface receptor signaling pathwaysearch defense response to Gram-negative bacteriumsearch negative regulation of interleukin-10 productionsearch positive regulation of NK T cell activationsearch positive regulation of T-helper 17 type immune responsesearch positive regulation of tyrosine phosphorylation of Stat3 proteinsearch negative regulation of inflammatory response to antigenic stimulussearch interferon-gamma biosynthetic processsearch positive regulation of smooth muscle cell apoptotic processsearch positive regulation of tumor necrosis factor productionsearch positive regulation of tyrosine phosphorylation of Stat5 proteinsearch cytokine-mediated signaling pathwaysearch sensory perception of painsearch positive regulation of NF-kappaB import into nucleussearch negative regulation of interleukin-17 productionsearch negative regulation of smooth muscle cell proliferationsearch extracellular spacesearch interleukin-12 complexsearch membranesearch cytoplasmsearch interleukin-23 complexsearch extracellular regionsearch

Chain InterPro annotation
A, C Interleukin-6/Interleukin-23/GCSF/MGFsearch Four-helical cytokine-like, coresearch Interleukin-23 alphasearch Four-helical cytokine, coresearch
B, D Long hematopoietin receptor, soluble alpha chain, conserved sitesearch Immunoglobulin subtype 2search Fibronectin type IIIsearch Immunoglobulin-like domainsearch Immunoglobulin-like foldsearch Interleukin-12 betasearch Interleukin-12 beta, central domainsearch