› 3d65

X-ray diffraction
1.64Å resolution

Crystal structure of Textilinin-1, a Kunitz-type serine protease inhibitor from the Australian Common Brown snake venom, in complex with trypsin

Released:
DOI: 10.2210/pdb3d65/pdb
PDB entry 3d65 coloured by chain, front view.
PDB entry 3d65 coloured by chain, side view.
PDB entry 3d65 coloured by chain, top view.
Source organisms:
Entry authors: Millers E-KI, Masci PP, Lavin MF, de Jersey J, Guddat LW

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: Arg-|-, Lys-|-.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133357 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Kunitz-type serine protease inhibitor textilinin-1 Chain: I
Molecule details ›
Chain: I
Length: 57 amino acids
Theoretical weight: 6.45 KDa
Source organism: Pseudonaja textilis textilis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q90WA1 (Residues: 27-83; Coverage: 97%)
Sequence domains: Kunitz/Bovine pancreatic trypsin inhibitor domain
Structure domains: Pancreatic trypsin inhibitor Kunitz domain
Serine protease 1 Chain: E
Molecule details ›
Chain: E
Length: 223 amino acids
Theoretical weight: 23.32 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00760 (Residues: 24-246; Coverage: 97%)
Gene names: PRSS1, TRP1, TRY1, TRYP1
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P3121
Unit cell:
a: 79.838Å b: 79.838Å c: 107.394Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.216 0.213 0.245
Expression system: Escherichia coli

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