› 3d5w

X-ray diffraction
2.6Å resolution

Crystal structure of a phosphorylated Polo-like kinase 1 (Plk1) catalytic domain in complex with ADP.

Released:
DOI: 10.2210/pdb3d5w/pdb
PDB entry 3d5w coloured by chain, front view.
PDB entry 3d5w coloured by chain, side view.
PDB entry 3d5w coloured by chain, top view.
Source organism: Danio rerio
Primary publication:
Structures of the wild-type and activated catalytic domains of Brachydanio rerio Polo-like kinase 1 (Plk1): changes in the active-site conformation and interactions with ligands.
Elling RA, Fucini RV, Romanowski MJ
Acta Crystallogr D Biol Crystallogr 64 909-18 (2008)
PMID: 18703838

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175633 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase PLK Chain: A
Molecule details ›
Chain: A
Length: 317 amino acids
Theoretical weight: 36.06 KDa
Source organism: Danio rerio
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4KMI8 (Residues: 1-312; Coverage: 52%)
Gene names: cb525, cb636, ik:tdsubc_2d1, plk, plk1, wu:fb37g11, wu:fb76g03, xx:tdsubc_2d1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ADP 1 x ADP
1 modified residue:
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: I23
Unit cell:
a: 133.99Å b: 133.99Å c: 133.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.244 0.242 0.271
Expression system: Escherichia coli

Quick links

Citations

1 review citation

PLK-1 Targeted Inhibitors and Their Potential against Tumorigenesis.
Kumar et al. (2015)
9 other articles

6 mentions without citation

On the molecular mechanisms of mitotic kinase activation.
Bayliss et al. (2012)
5 more