› 3d4p

X-ray diffraction
1.8Å resolution

Crystal structure of Lactate Dehydrogenase from Staphylococcus Aureus complexed with NAD and pyruvate

Released:
DOI: 10.2210/pdb3d4p/pdb
PDB entry 3d4p coloured by chain, front view.
PDB entry 3d4p coloured by chain, side view.
PDB entry 3d4p coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus COL
Entry authors: Schramm VL, Almo SC, Gutierrez JA, Ho M

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177208 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 317 amino acids
Theoretical weight: 34.62 KDa
Source organism: Staphylococcus aureus subsp. aureus COL
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5HJD7 (Residues: 1-317; Coverage: 100%)
Gene names: SACOL0222, ldh1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
1 bound ligand:
Ligand PYR 2 x PYR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 130.868Å b: 74.916Å c: 95.874Å
α: 90° β: 128.9° γ: 90°
R-values:
R R work R free
0.164 0.163 0.188
Expression system: Escherichia coli

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