3cvs Summary

pdbe.org/3cvs
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Crystal Structure of an AlkA Host/Guest Complex 8oxoGuanine:Adenine Base Pair

The structure was published by Bowman, B.R., Lee, S., Wang, S., and Verdine, G.L., in 2008 in a paper entitled "Structure of the E. coli DNA Glycosylase AlkA Bound to the Ends of Duplex DNA: A System for the Structure Determination of Lesion-Containing DNA." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.4 Å and deposited in 2008.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 3 biomacromolecules, namely DNA-3-methyladenine glycosylase 2, DNA (5'-D(*DGP*DAP*DCP*DAP*DTP*DGP*DAP*(8OG)P*DTP*DGP*DCP*DC)-3'), and DNA (5'-D(*DGP*DGP*DCP*DAP*DAP*DTP*DCP*DAP*DTP*DGP*DTP*DC)-3').

The molecule most likely forms heterooctamers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A DNA-3-methyladenine glycosylase 2 P04395 (1-282) (3MG2_ECOLI)search Escherichia coli K-12search 100% 282 100%
B DNA-3-methyladenine glycosylase 2 P04395 (1-282) (3MG2_ECOLI)search Escherichia coli K-12search 100% 282 100%
C DNA-3-methyladenine glycosylase 2 P04395 (1-282) (3MG2_ECOLI)search Escherichia coli K-12search 100% 282 100%
D DNA-3-methyladenine glycosylase 2 P04395 (1-282) (3MG2_ECOLI)search Escherichia coli K-12search 100% 282 100%


This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
P04395 (1 - 282) DNA-3-methyladenine glycosylase 2 Escherichia coli

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A, B, C, D (P04395) DNA repair glycosylase, 2 C-terminal domainssearch, DNA repair glycosylase, N-terminal domainsearch DNA-3-methyladenine glycosylase iisearch, Hypothetical protein; domain 2search, Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)search PF00730: HhH-GPD superfamily base excision DNA repair proteinsearch, PF06029: AlkA N-terminal domainsearch

Chain ID Biological process (GO) Molecular function (GO) Cellular component (GO)
A, B, C, D (P04395) DNA repairsearch base-excision repairsearch DNA dealkylation involved in DNA repairsearch cellular response to DNA damage stimulussearch alkylbase DNA N-glycosylase activitysearch catalytic activitysearch DNA-3-methyladenine glycosylase activitysearch DNA-3-methylguanine glycosylase activitysearch hydrolase activitysearch DNA-7-methylguanine glycosylase activitysearch DNA-7-methyladenine glycosylase activitysearch cytoplasmsearch

Chain InterPro annotation
A, B, C, D Alkylbase DNA glycosidase, conserved sitesearch HhH-GPD domainsearch DNA-3-methyladenine glycosylase AlkA, N-terminalsearch DNA glycosylasesearch Helix-turn-helix, base-excision DNA repair, C-terminalsearch