› 3csh

X-ray diffraction
1.55Å resolution

Crystal Structure of Glutathione Transferase Pi in complex with the Chlorambucil-Glutathione Conjugate

Released:
DOI: 10.2210/pdb3csh/pdb
PDB entry 3csh coloured by chain, front view.
PDB entry 3csh coloured by chain, side view.
PDB entry 3csh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The anti-cancer drug chlorambucil as a substrate for the human polymorphic enzyme glutathione transferase P1-1: kinetic properties and crystallographic characterisation of allelic variants.
Parker LJ, Ciccone S, Italiano LC, Primavera A, Oakley AJ, Morton CJ, Hancock NC, Bello ML, Parker MW
J Mol Biol 380 131-44 (2008)
PMID: 18511072

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140593 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase P Chains: A, B
Molecule details ›
Chains: A, B
Length: 209 amino acids
Theoretical weight: 23.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09211 (Residues: 2-210; Coverage: 100%)
Gene names: FAEES3, GST3, GSTP1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand GSH 2 x GSH

Cofactor: Ligand LZ6 2 x LZ6
4 bound ligands:
Ligand CL 2 x CL
Ligand MES 2 x MES
Ligand CA 1 x CA
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: C2
Unit cell:
a: 77.346Å b: 89.358Å c: 68.946Å
α: 90° β: 98.03° γ: 90°
R-values:
R R work R free
0.18 0.17 0.2
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Glutathione transferases: a structural perspective.
Oakley A. (2011)
3 more

2 mentions without citation

The interaction of the chemotherapeutic drug chlorambucil with human glutathione transferase A1-1: kinetic and structural analysis.
Karpusas et al. (2013)
1 more