› 3crr

X-ray diffraction
1.9Å resolution

Structure of tRNA Dimethylallyltransferase: RNA Modification through a Channel

Released:
DOI: 10.2210/pdb3crr/pdb
PDB entry 3crr coloured by chain, front view.
PDB entry 3crr coloured by chain, side view.
PDB entry 3crr coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structure of tRNA dimethylallyltransferase: RNA modification through a channel.
Xie W, Zhou C, Huang RH
J Mol Biol 367 872-81 (2007)
PMID: 17292915

Function and Biology Details

Reaction catalysed: 
Dimethylallyl diphosphate + adenine(37) in tRNA = diphosphate + N(6)-(3-methylbut-2-en-1-yl)-adenine(37) in tRNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190967 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA dimethylallyltransferase Chain: A
Molecule details ›
Chain: A
Length: 323 amino acids
Theoretical weight: 35.84 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HUL9 (Residues: 1-323; Coverage: 100%)
Gene names: PA4945, miaA
Sequence domains: IPP transferase
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand MG 1 x MG
Ligand CL 1 x CL
Ligand DPO 1 x DPO
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P41
Unit cell:
a: 76.23Å b: 76.23Å c: 57.809Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.209 0.235
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

Evolution of cytokinin biosynthesis and degradation.
Frébort et al. (2011)
4 more