› 3coq

X-ray diffraction
2.4Å resolution

Structural Basis for Dimerization in DNA Recognition by Gal4

Released:
DOI: 10.2210/pdb3coq/pdb
PDB entry 3coq coloured by chain, front view.
PDB entry 3coq coloured by chain, side view.
PDB entry 3coq coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for dimerization in DNA recognition by Gal4.
Hong M, Fitzgerald MX, Harper S, Luo C, Speicher DW, Marmorstein R
Structure 16 1019-26 (2008)
PMID: 18611375

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116149 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Regulatory protein GAL4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 89 amino acids
Theoretical weight: 10.48 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P04386 (Residues: 8-96; Coverage: 10%)
Gene names: GAL4, YPL248C
Sequence domains:
Structure domains: Zn(2)-C6 fungal-type DNA-binding domain
DNA (5'-D(*DAP*DCP*DCP*DGP*DGP*DAP*DGP*DGP*DAP*DCP*DAP*DGP*DTP*DCP*DCP*DTP*DCP*DCP*DGP*DG)-3') Chain: D
Molecule details ›
Chain: D
Length: 20 nucleotides
Theoretical weight: 6.14 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*DTP*DCP*DCP*DGP*DGP*DAP*DGP*DGP*DAP*DCP*DTP*DGP*DTP*DCP*DCP*DTP*DCP*DCP*DGP*DG)-3') Chain: E
Molecule details ›
Chain: E
Length: 20 nucleotides
Theoretical weight: 6.13 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MPD 1 x MPD
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25, CHESS BEAMLINE F1
Spacegroup: C2
Unit cell:
a: 126.495Å b: 40.829Å c: 90.418Å
α: 90° β: 95.88° γ: 90°
R-values:
R R work R free
0.213 0.207 0.269
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Genome-wide analysis of the Zn(II)₂Cys₆ zinc cluster-encoding gene family in Aspergillus flavus.
Chang et al. (2013)
2 more

23 mentions without citation

ConSurf 2010: calculating evolutionary conservation in sequence and structure of proteins and nucleic acids.
Ashkenazy et al. (2010)
22 more