3cn9

X-ray diffraction
2.09Å resolution

Crystal Structure Analysis of the Carboxylesterase PA3859 from Pseudomonas aeruginosa PAO1- orthorhombic crystal form

Released:

Function and Biology Details

Reaction catalysed:
A carboxylic ester + H(2)O = an alcohol + a carboxylate
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-191103 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospholipase/carboxylesterase/thioesterase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 226 amino acids
Theoretical weight: 24.72 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HXE7 (Residues: 2-215; Coverage: 100%)
Gene name: PA3859
Sequence domains: Phospholipase/Carboxylesterase
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P21212
Unit cell:
a: 96.423Å b: 96.363Å c: 68.044Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 0.24
Expression system: Escherichia coli