› 3cev

X-ray diffraction
2.4Å resolution

Function and Biology Details

Reaction catalysed: 
L-arginine + H(2)O = L-ornithine + urea
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo hexamer
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156819 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arginase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 299 amino acids
Theoretical weight: 32.48 KDa
Source organism: [Bacillus] caldovelox
Expression system: Escherichia coli
UniProt:
  • Canonical: P53608 (Residues: 1-299; Coverage: 100%)
Gene name: rocF
Sequence domains: Arginase family
Structure domains: Ureohydrolase domain

Ligands and Environments

2 bound ligands:
Ligand MN 6 x MN
Ligand ARG 12 x ARG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2221
Unit cell:
a: 106.8Å b: 277.7Å c: 140Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.193 0.243
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Emerging themes in manganese transport, biochemistry and pathogenesis in bacteria.
Kehres et al. (2003)
4 more

4 mentions without citation

Replacing Mn(2+) with Co(2+) in human arginase i enhances cytotoxicity toward l-arginine auxotrophic cancer cell lines.
Stone et al. (2010)
3 more